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Millipore This Anti-Muscarinic Acetylcholine Receptor M4 Antibody, Clone 17f10.2 Is Validated For Use In Ic, Ip, Wb For The Detection Of Muscarinic Acetylcholine Receptor M4. - Mill (Additional S&H or Hazmat Fees May Apply)
Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH=7.6 with 0.1% sodium azide.
Quality Level
MQ100
Applications
Application
This Anti-Muscarinic Acetylcholine Receptor m4 Antibody, clone 17F10.2 is validated for use in IC, IP, WB for the detection of Muscarinic Acetylcholine Receptor m4.
Key Applications
Immunocytochemistry
Immunoprecipitation
Western Blotting
Application Notes
Immunohistochemistry (rat and monkey)
Western blot (rat, mouse, human): ECL 4-10 μg/mL
Immunoprecipitation (rat)
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
m4 receptor i3 loop (human), fused to GST
Clone
17F10.2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
m4 muscarinic acetylcholine receptor. No reactivity with the other subtypes.
Isotype
IgG1
Species Reactivity
Mouse
Monkey
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NM_000741.2
Entrez Gene Summary
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition.
Gene Symbol
CHRM4
HM4
UniProt Number
P08173
UniProt Summary
FUNCTION: SwissProt: P08173 # The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase. SIZE: 479 amino acids; 53049 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P08173 ## Belongs to the G-protein coupled receptor 1 family.
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at 2-8°C in undiluted aliquots for up to 6 months.
