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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1586 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Affinity purified immunoglobulin. Lyophilized. Reconstitute with 50 μL of PBS, pH 7.4. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect NMDAR1 using this Anti-NMDAR1 Antibody, (all splice variants), clone R1JHL validated for use in WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:300. It is recommended that the antibody be diluted in TBST (20 mM Tris-HCl, 137 mM NaCl, 1% Tween-20, pH 7.6) containing 5% nonfat milk and 0.05% sodium azide. The diluted antibody can be used at least 5 times without a significant signal decrease. Store at 2-8°C between uses. Immunoprecipitation: 3 μg per 200 μg of lysate will quantitatively precipitate all the NMDA R1 in 200 μg of rat brain lysate. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Fusion protein of amino acids 1-564 from rat NR1. |
| Epitope | (all splice variants) |
| Clone | R1JHL |
| Host | Mouse |
| Specificity | NMDA receptor subunit 1 (all splice variants). No cross-reactivity observed with other NMDA receptor subunits. Recognizes a 103-116 kDa protein in rat brain hippocampal homogenate by immunoblot. |
| Isotype | Ig |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity). SIZE: 938 amino acids; 105373 Da SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity). SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity). PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity). SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain lyophilized material at -20°C for up to six months after date of receipt. After reconstitution, maintain at -20°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 15 µg |