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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC | M | Phycoerythrin | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1794H |
| Replaces | CBL141P |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated |
| Overview | BIOCHEMISTRY: p I = 7.0 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Phycoerythrin |
| Presentation | Purified from mouse ascitic fluid. R-Phycoerythrin conjugated. The antibody is supplied in 1.0ml PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by electrophoresis and flow cytometry. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD19 using this Anti-Human B cells (CD19) Antibody, clone FMC63, Phycoerythrin conjugated validated for use in FC. |
| Key Applications |
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| Application Notes | This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry. SUGGESTED USAGE Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E(6) peripheral blood mononuclear cells (PBMC) in 100 μl buffer. |
| Biological Information | |
|---|---|
| Immunogen | Raji cells |
| Clone | FMC63 |
| Host | Mouse |
| Specificity | This specificity of this antibody is equivalent to that of CD19 monoclonal antibodies (Zola et al., 1988, 1989), based on comparative studies, antigen recognition and positive reaction with a CD19 transfected cell line. It detects a 96 kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. The antibody reacts with most B cell malignancies including chronic lymphocytic leukemia and most lymphomas, but not multiple myeloma. Cell reactivity: Normal:Stains all B lymphocytes in peripheral blood and spleen. Negative on granulocytes, monocytes, platelets, erythrocytes and T lymphocytes. Clinical: B cell chronic lymphocytic leukemia (B-CLL) + Pro lymphocytic leukemia (PLL + Hairy cell leukemia (HCL) + Common acute lymphoblastic leukemia (CALL) + Pre-B acute lymphoblastic leukemia (pre-B-ALL) + NULL-acute lymphoblastic leukemia (NULL-ALL) + T-acute lymphoblastic leukemia (T-ALL) - T-common lymphocytic leukemia (T-CLL - |
| Isotype | IgG2a |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P15391 # Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. SIZE: 556 amino acids; 61128 Da SUBUNIT: Forms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE:SwissProt: P15391 # Defects in CD19 are a cause of hypogammaglobulinemia [MIM:107265]. SIMILARITY: SwissProt: P15391 ## Contains 2 Ig-like C2-type (immunoglobulin-like) domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water. |
| Packaging Information | |
|---|---|
| Material Size | 100 assays |