IBI Scientific DNA/RNA/Protein Extraction Kit

IBI Scientific DNA/RNA/Protein Extraction Kit, Up to 9 ug of Genomic DNA, 20 ug of Total RNA, 120 ug of Protein from 1.5 x 106 HeLa Cells, Spin Column Format, DNA/RNA Purication within 25 min, Protein Precipitation within 50 min Processing Time, 50 Preparations Per Kit. The DNA RNA protein extraction kit provides an efficient method for purifying genomic DNA, total RNA and total protein simultaneously from cultured cells, animal tissues, whole blood and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water. DNA/RNA purification can be completed in 25 min without phenol/chloroform extraction or alcohol precipitation and protein purification can be completed in 50 min. The purified DNA, with approximately 20 to 30 Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot. The quality of the DNA RNA protein extraction kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1 x 106 cultured animal cells. The purified DNA and total RNA is quanti-fied with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quanti-fied by Bradford assay analyzed on SDS-PAGE - IBI (Additional Shipping And Hazmat May Apply)