Takara RiboGone - Mammalian \24 Rxns

RiboGone - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S,and28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA. This kit is designed for use with limited sample amounts (10–100 ng of total RNA), and works with high- or low-quality RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with random primers.

RiboGone - Mammalian removes rRNA efficiently from intact and degraded RNA, while retaining noncoding transcripts for analysis.RNA-seq libraries were generated from Human Brain Total RNA (Takara Bio) or Breast Cancer FFPE RNA (Cureline, extracted using a NucleoSpin totalRNA FFPE kit) and treated with the indicated rRNA removal method. Reads were mapped to the hg19 genome and read distributions were determined using Picard RNA-Seq Metrics. Libraries generated from RiboGone-treated RNA had comparably low rRNA reads to oligo(dT)-enriched RNA (Takara Bio), while retaining more noncoding reads.