Takara Marathon cDNA Amplification Kit \100 Rxns

This kit contains reagents for making adaptor-ligated ds cDNA from poly A+ RNA and performing 5 and 3 Marathon RACE PCR using gene-specific primers and a mixture of thermostable DNA polymerases supplied by the user. This kit contains sufficient reagents for 5 cDNA synthesis reactions and 100 PCR reactions.

Marathon cDNA amplification of abundant (actin, 1.9 kb) and moderately rare (TFR, 5.1 kb) transcripts.The 5- and 3-RACE reactions for actin and TFR were performed with adaptor-ligated ds cDNA made from 1 μg of human placental poly A+RNA and amplified for 25 PCR cycles. Full-length cDNAs were end-to-end PCR–amplified according to the User Manual. Lane 1: 1.2-kb actin 5-RACE product. Lane 2: 1.3-kb actin 3-RACE product. Lane 3: full-length 1.9-kb actin cDNA. Lane 4: 2.6- kb TFR 5-RACE product. Lane 5: 2.9-kb TFR 3-RACE product. Lane 6: full-length 5.1-kb TFR cDNA. Lane m: 1-kb DNA size ladder.