Beckman Lineage-Pe Cocktail, 100 Tests, Ruo

The Lineage-PE Cocktail is comprised of PE-conjugated CD3, CD14, CD19, CD20 and CD56 antibodies. It enables the positive staining of five immune cell populations (T and B Lymphocytes, NK cells, Monocytes/Macrophages) together, without distinction, in the PE channel using flow cytometry. When combined with fluorescent-labeled antibodies, other than PE, the gating on PE negative cells allows the phenotypical identification of Dendritic cells. The CD20 molecule is expressed on B lineage cells. Its CD20 expression occurs early in pre-B lymphocyte development, persists throughout B lymphocyte ontogeny and is lost upon ultimate plasma cells differentiation. The CD20 molecule is present on all B lymphocytes whatever the hematopoietic tissue where they are found (peripheral blood, lymph nodes, spleen, tonsil, or bone marrow). CD20 is also expressed, but at low density, on a subset of peripheral blood T lymphocytes. The CD56 molecule, known as NCAM (Neural Cell Adhesion Molecule) antigen, is expressed on a subpopulation of peripheral blood large granular lymphocytes and on all cells with natural killer (NK) activity. It is also expressed by a minor subpopulation of CD3+ T cells that mediates reduced cytotoxic activity. It is not found on monocytes, granulocytes, erythrocytes or B lymphocytes. The CD20 molecule is expressed on B lineage cells. Its CD20 expression occurs early in pre-B lymphocyte development, persists throughout B lymphocyte ontogeny and is lost upon ultimate plasma cells differentiation. The CD20 molecule is present on all B lymphocytes whatever the hematopoietic tissue where they are found (peripheral blood, lymph nodes, spleen, tonsil, or bone marrow). CD20 is also expressed, but at low density, on a subset of peripheral blood T lymphocytes. The CD56 molecule, known as NCAM (Neural Cell Adhesion Molecule) antigen, is expressed on a subpopulation of peripheral blood large granular lymphocytes and on all cells with natural killer (NK) activity. It is also expressed by a minor subpopulation of CD3+ T cells that mediates reduced cytotoxic activity. It is not found on monocytes, granulocytes, erythrocytes or B lymphocytes.