Revvity Nextflex 16s V3 &Ndash; V4 Amplicon-Seq Library Prep Kit

• Optimized protocol offers lower PCR bias and fewer off-target reads
• Fast library prep protocol
• Low input – As low as 1 ng of genomic DNA
• Flexible barcode options
• Automation protocols are now available for the Revvity Sciclone® NGSx Workstation to automate your 16S sequencing
• Functionally tested on the Illumina^® MiSeq^® sequencer

Optimized 16S Protocol Offers Lower PCR Bias and Fewer Off-target Reads

The protocol incorporated in the NEXTFLEX 16S V3 – V4 Amplicon-Seq Kit offers better sequencing results than can be obtained using traditional 16S sequencing protocols. The incorporation of the second PCR step in the protocol for the addition of the sample-specific index reduces the number of off-target reads typically encountered during amplicon sequencing.

Increase 16S V3 – V4 throughput and reduce errors with automation

A NEXTFLEX^® automation protocol is now available on the Sciclone NGSx workstations to help labs increase their throughput and reduce human errors. Automated versions of the NEXTFLEX 16S V3 – V4 Amplicon-Seq Library Prep kits are available by request.