Sigma-Aldrich Quantitative RT-qPCR Kit

Sigma-Aldrich Quantitative RT-qPCR Kit, Detection Method: SYBR Green, Number of Reactions: 250, Colorless, -20 deg C. The SYBR green quanti-tative RT-PCR kit combines Maloney murine leukemia virus reverse transcriptase (M-MLV RT), JumpStartTaq DNA polymerase and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStartTaq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator and stabilizers. The JumpStartTaq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70 deg C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques - SIAL (Additional S&H Or Hazmat Fees May Apply)