Takara PrimeScript RT-PCR Kit \50 Rxns

This kit for two-step RT-PCR is designed to provide excellent extendibility and efficient amplification. It uses PrimeScript reverse transcriptase and TakaraEx TaqHS polymerase. This kit generates RT-PCR products efficiently at standard reverse transcription temperature (42℃), even when RNA templates contain higher-order structures. It is not necessary to perform reactions at a high temperature when there is a risk of RNA decomposition. This kit includes all reagents necessary for the reverse transcription of RNA to cDNA, and for cDNA amplification using PCR.

RT-PCR using various amounts of RNA template.To illustrate the high sensitivity of the PrimeScript™ RT-PCR Kit in detecting mRNAs in very low quantities of total RNA template, GAPDH mRNA was used as a target in various amounts of HL60 cell total RNA (0.1 pg to 1 ng). The RNA was reverse transcribed using the oligo dT primer. The kit protocol was followed for RT and PCR.

Amplifying various lengths of target gene mRNAs by RT-PCR.Using human heart total RNA or HL60 cell total RNA as a template, a variety of sequence lengths from two target gene mRNAs (dystrophin and transferrin receptor) were reverse transcribed and amplified by PCR using the reagents in the PrimeScript™ RT-PCR Kit.

Principle of PrimeScript RT-PCR Kit.