Takara TaKaRa LA Taq DNA Polymerase with GC Buffer \125 Units

TaKaRa LA Taq DNA Polymerase (Long andAccurate) with GC Buffer is a blend of TaKaRa Taq and a proofreading polymerase, supported by two GC-optimized buffers for amplification of long, GC-rich templates. Includes separate tubes of TaKaRa LA Taq DNA Polymerase, GC Buffer I, GC Buffer II, and dNTPs.

Comparison of TakaraLA Taqwith GC Buffer and TakaraLA TaqDNA polymerase for amplification of a GC-rich target fragment.A 2.1 kb mouse rRNA gene (63.4% GC) was amplified from of mouse genomic DNA. TakaraLA Taqwith GC Buffer resulted in high yield, high specificity amplification.

Lanes:M1: lambda-Hind III digest1 and 2:LA Taq/ LA PCR Buffer II3 and 4:LA Taqwith GC Buffer / GC Buffer I5 and 6:LA Taqwith GC Buffer / GC Buffer IIM2: pHY Marker

Amplification of the Huntingtons Disease (HD) gene usingTakara LA TaqDNA Polymerase.The HD gene is GC-rich and highly repetitive and the primers used in this experiment amplified regions of the HD gene IT15 CAG repeat. For all reactions, purified human genomic DNA (100 ng in a 50 µL reaction) was used as a template in reactions containingTakara LA Taqpolymerase with either LA PCR Buffer II (lane 1), GC Buffer I (lane 2), or GC Buffer II (lane 3), or a competitors high GC DNA polymerase kit (lane 4). The sizes of the amplified products were 262 bp (GC content 73%) and 358 bp (GC content 71.5%). Lane m contains a 100 bp ladder.