| Description | |
|---|---|
| Catalogue Number | SCR727 |
| Description | B18R-E3L Plasmid (human codon optimized for B18R and E3L) |
| Background Information | Simplicon® is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome. By nature of a synthetic and self-replicative RNA, interferon (IFN) responses occur at the transfection and replication of Simplicon® RNA. The IFN responses reduce the expression and lifetime of Simplicon® RNA. Therefore, it is important to suppress the IFN responses in Simplicon® System. B18R acts as a decoy receptor for Type I Interferons and inhibit IFN-alpha, beta, and omega and other Type I IFN receptors. When only employing B18R, some IFN-beta response still occurs. The vaccinia virus E3L gene encodes two double-stranded RNA binding proteins that suppressed IFN-beta response and improved the expression of Simplicon® RNA at RNA transfection and continuous cell culture. In product B18R-E3L plasmid (human codon optimized for B18R and E3L), B18R and E3L are provided as a polycistronic DNA that can be transcribed into a polycistronic mRNA to be co-transfected with Simplicon® RNA(s) for IFN suppression. For continuous expression, B18R protein is needed to supply with B18R-conditioned medium (B18R-cm) or recombinant protein. |
| Applications | |
|---|---|
| Application | To strongly suppress more interferon responses than single B18R to enable more expression of a Simplicon® RNA or regular mRNA(s). |
| Application Notes | B18R-E3L Plasmid (human codon optimized for B18R and E3L) is provided as a polycistronic DNA with both the B18R and E3L genes to more strongly suppress the interferon (IFN) responses that degrades Simplicon® RNA(s) or regular mRNA(s). Upon transcription, B18R RNA (E3L) works at single co-transfection with Simplicon® RNA or repeated co-transfection with regular mRNA(s). B18R RNA (E3L) can also be used for production of B18R-cm (B18R-conditioned medium). |
| Biological Information | |
|---|---|
| Concentration | Please refer to lot specific datasheet. |
| Product Usage Statements | |
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| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | B18R-E3L Plasmid (Cat. # SCR727). One (1) vial containing 10 µL of Plasmid (1 µg/µL). Store at -20 °C |
| Packaging Information | |
|---|---|
| Material Size | 1 vial |