Takara Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1) \Each

The Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1) is a tetracycline-inducible lentiviral gene expression system that allows you to produce high titers of recombinant, VSV-g pseudotyped lentiviruses for the purpose of establishing a tightly inducible expression system in both dividing and non-dividing cell types. pLVX-TRE3G-ZsGreen1 contains an internal ribosome entry site (IRES), allowing the simultaneous induced expression of a gene of interest and a green fluorescent protein markerfrom the same RNA transcript. The pLVX-EF1a-Tet3G Vector expresses the Tet-On 3G transactivator protein from the human EF1 alpha promoter, allowing long-term expression of the transactivator in a broad range of cell types, including those in which a CMV promoter can be silenced over time, such as hematopoietic stem cells. The kit also includes a complete fourth generation lentiviral packaging system, Lenti-X Packaging Single Shots (VSV-g).

Lentiviral Tet-On 3G systems.There are several systems available that express the Tet-On 3G transactivator from the human EF1-alpha promoter. These systems are particularly recommended for establishing tetracycline inducible expression in cell types known to silence the CMV promoter over time, such as stem cells or hematopoietic cells..

Required Products

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems. This FBS has been processed in the United States.

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems.Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems.Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.

Fetal Bovine Serum (FBS) that has been functionally tested for optimal use with all of our Tet Systems. This FBS does not contain trace levels of tetracycline (or its derivatives) which have been observed to interfere with proper regulation of TRE-controlled gene expression. Use of this serum ensures the maximal range of induction possible with the Tet Systems.

Fetal bovine serum (FBS) approved for use with tetracycline inducible expression systems.Only Takara Bio USA functionally tests FBS to be sure that no contaminating tetracycline derivatives interfere with induced expression levels.

Fetal bovine serum from Clontech is functionally tested to guarantee consistent results with tetracycline-inducible expression systems.Serum from other vendors that have not been functionally tested may contain tetracyclines that affect the maximum expression in Tet-Off systems, or generate background in Tet-On systems. Data here shows induction of luciferase expression from our CHO-AA8-Luc Tet-Off Control Cell Line using different sources of FBS.