Takara Recombinant RNase Inhibitor ver.2.0

Recombinant RNase Inhibitor ver.2.0 contains a mutation in a cysteine residue that is very sensitive to oxidation, making it highly stable in enzymatic reactions requiring low DTT concentrations. It inhibits RNase A activity through the formation of a 1:1 complex in a reversible reaction. It is not effective against RNase H activity.

Comparison of oxidation resistance among different commercially-available recombinant RNase inhibitors.16S/23S rRNA was mixed with DTT-free buffer, RNase A, increasing amounts of H2O2, and either Recombinant RNase Inhibitor ver.2.0 (Cat. # 2135; purple line), Recombinant RNase Inhibitor (Cat. # 2313; blue line), or a commercially-available, oxidation-resistant murine RNase inhibitor (Company N; green line). After incubation at 37°C for 10 min followed by incubation at 25°C for 10 min, each reaction was centrifuged at 13,000gfor 15 min at 4°C. Nucleic acids released into the supernatant were measured as an indicator of RNase A-mediated rRNA degradation using a NanoDrop spectrophotometer. At concentrations above 0.1% H2O2, the use of Recombinant RNase Inhibitor ver.2.0 resulted in less rRNA degradation compared to other RNase inhibitor products.n=2, where each experiment was performed in triplicate.