Takara SMART-Seq® Total RNA Pico Input with UMIs (ZapR™ Mammalian), 24 Rxns

SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) generates strand-specific RNA-seq libraries for Illumina sequencing from 250 pg–1 µg inputs of purified total RNA or from 10–1,000 intact cells. The library design featured in this kit adds an 8 nucleotide (nt) unique molecular identifier (UMI) through the reverse-transcription step to mitigate potential PCR bias as well as to provide additional information for transcript quantification. Enough material is provided with this kit to perform up to 24 reactions.

Ribosomal rRNA depletion and gene and transcript counts for human brain total RNA-seq libraries. SMART-Seq Total RNA Library Prep (with UMIs) was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 3 x 106paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 250 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 250 pg-input untreated libraries.

Transcript expression correlation across input amounts for human brain total RNA-seq libraries. SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) was used to prepare libraries from 250 pg and 10 ng of human brain RNA. Data analysis was performed with CogentAP using 3 x 106paired-end reads. The scatterplot illustrates the high correlation between the 10 ng-input libraries and 250 pg-input libraries.

Ribosomal rRNA depletion and gene and transcript counts for primary B-cell total RNA-seq libraries. SMART-Seq Total RNA Library Prep (with UMIs) was used to prepare libraries from 1 ng of human primary B-cell RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 3 x 106paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution for libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to untreated libraries.

Transcript expression correlation across replicates for primary B-cell RNA-seq libraries. SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) was used to prepare libraries from 1 ng of human primary B-cell RNA. Data analysis was performed with CogentAP using 3 x 106paired-end reads. The scatterplot illustrates the high correlation between the two replicates.

Ribosomal rRNA depletion and gene and transcript counts for libraries produced from degraded FFPE RNA samples. SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs) was used to prepare libraries from 10 ng of FFPE RNA (RIN = 3, DV200= 77%). Data analysis was performed with CogentAP using 3 x 106paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution for two replicates.

Improved rRNA depletion and enhancement of biologically relevant reads over original SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian. SMART-Seq Total RNA Library Prep (with UMIs) was used to prepare libraries from 250 pg of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs)) and enriched through PCR amplification or left untreated. In addition, SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian was used to prepare libraries from 250 pg of human brain RNA. Data analysis was performed with CogentAP using 3 x 106paired-end reads. The read distribution of libraries treated with the ZapR Mammalian rRNA Depletion Kit shows a decreased percentage of rRNA-associated reads and an increased percentage of exonic reads compared to libraries prepared using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian.

Ribosomal rRNA depletion and gene and transcript counts for mouse brain total RNA-seq libraries. SMART-Seq Total RNA Library Prep (with UMIs) was used to prepare libraries from 250 pg of mouse brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Library Prep with ZapR Depletion (with UMIs)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 3 x 106paired-end reads. Read distribution (Panel AandPanel C) and gene count (Panel BandPanel C) are shown for libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to untreated libraries.

Required Products

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.