Takara SMART-Seq® Total RNA Single Cell (ZapR™ Mammalian) \ 24 Rxns

SMART-Seq Total RNA-Seq Single Cell (ZapR Mammalian) generates strand-specific RNA-seq libraries for Illumina sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. The kit was specifically designed to deliver highly sensitive and reproducible data from single cells while keeping the workflow short and user friendly. The kit does not require additional rRNA removal methods or kits and produces sequencing libraries that retain strand-of-origin information. Enough material is provided with this kit to perform up to 24 reactions.

Efficient depletion of rRNA-derived cDNAs across a wide input range. SMART-Seq Total RNA Single Cell was used to prepare libraries from 10 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Single Cell (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. The bar graph (Panel A) and table (Panel B) show the read distribution of 10 pg-input and 10 ng-input libraries treated with the ZapR Mammalian rRNA Depletion Kit compared to 10 pg-input and 10 ng-input untreated libraries.

Superior gene and transcript detection across a wide input range. SMART-Seq Total RNA Single Cell was used to prepare libraries from 10 pg and 10 ng of human brain RNA. Libraries were then either treated with the ZapR Mammalian rRNA Depletion Kit (sold as part of SMART-Seq Total RNA Single Cell (ZapR Mammalian)) and enriched through PCR amplification or left untreated. Data analysis was performed with CogentAP using 2 x 106paired-end reads. Gene counts (Panel A) and gene and transcript counts (Panel B) are shown for the ZapR-treated and untreated 10 pg-input and 10 ng-input libraries.

Required Products

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.