Takara SMART-Seq® mRNA Single Cell LP

The SMART-Seq mRNA Single Cell LP Kit is a complete kit designed to first generate high-quality cDNA from single cells and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit.This kit supports up to 96 reactions.

Comparing full-volume versus eighth-volume processing on the mosquito HV with single CHO Cells.Boxplots of gene counts for each preparation (medians: FV=16,592, Eighth=16,014). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

Clustering of the SSsc single cell and positive controls and significantly distinct discrimination of the negative controls provides confidence in the data generated with SSsc PLUS.By UMAP analysis, there is a clear separation between the negative controls and the positive controls/single-cell samples for SSsc PLUS. As expected, the positive controls (grey) and the single-cell samples (green) cluster together for the SSsc PLUS while the negative controls (dusty blue) cluster together near the bottom of the chart. Interestingly, the SSsc PLUS negative controls and all of the NEBNext samples cluster together: negative (red), positive (light blue), and the single cells (purple). These analyses indicate that experimental noise, even when working with such small amounts of starting material, will not affect confidence in the biological import of the results. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

Comparing full-volume versus quarter-volume processing with single GM12878 cells on the MANTIS Liquid Dispenser.Boxplots of gene counts for each preparation (medians: FV=9,980, Quarter=9,603). Boxplots show similar sensitivity for FV versus miniaturized volumes. All boxplots interquartile range (IQR) is the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SMART-Seq Single Cell.

Boxplots representing the distribution of gene counts for TPM > 0.1.The boxes denote the interquartile range (IQR), i.e., the 25th and 75th quartiles; the whiskers are 1.5X IQR from the median value and represent the extremes of the data. Outliers are plotted as empty circles. Data is shown for SMART-Seq Single Cell (SSsc). SMART-Seq mRNA Single Cell is an equivalent replacement for SSsc.

Comparable data between SMART-Seq library prep and Nextera XT kits enable existing users to easily make the switch.Representative XY-plots of two example libraries from the same cDNA prepared with SSsc PLUS or Nextera XT in duplicate, and the same cDNA library compared between Nextera XT and PLUS. R2values are shown in the graph; Nextera XT R2=0.9961, PLUS R2=0.9978, Nextera XT vs PLUS R2=0.9579. Data is shown for SMART-Seq Single Cell PLUS (SSsc PLUS). SMART-Seq mRNA Single Cell LP is an equivalent replacement for SSsc PLUS.

Required Products

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.