Takara SMART-Seq® mRNA HT LP

SMART-Seq mRNA HT LP is a complete kit designed to first generate high-quality cDNA from 1–100 cells or ultra-low amounts of high-integrity total RNA (10 pg–1 ng) and then high-quality Illumina sequencing-ready libraries. Indexes are added using a unique dual index kit.This kit supports up to 384 reactions.

Read distribution of SMART-Seq mRNA HT LP compared to Nextera XT.SMART-Seq mRNA HT LP was used to produce cDNA from 10 pg of Mouse Brain Total RNA in triplicate. Illumina-compatible libraries were generated from 1 ng or 125 pg using the SMART-Seq Library Prep Kit portion of the SMART-Seq mRNA HT LP or Nextera XT (Illumina) library prep kits, respectively, and the libraries were sequenced on a NextSeq 500 system. The reads were normalized to 4 m paired-end reads and analyzed using CLC Genomic Workbench. The distribution of mapped reads was similar between the two library preparation methods.

Gene identification performance of SMART-Seq mRNA HT LP compared to Nextera XT.Libraries were prepared in duplicate from the same SMART-Seq mRNA HT LP cDNA using either the SMART-Seq Library Prep Kit portion of SMART-Seq mRNA HT LP or Nextera XT.Panel A.Correlation plots between libraries made with SMART-Seq mRNA HT LP and Nextera XT kits. Pearson and Spearmans correlations were calculated from the TPM values obtained. Scatter plots show the TPM values from all genes with a log10+1 scale.Panel B.Venn diagram showing the number of genes identified from the processed cDNA, in duplicate, with SMART-Seq mRNA HT LP, Nextera XT, or between both chemistries. Both methods detected the same 12,311 genes at similar TPM values.

Required Products

The Unique Dual Index (UDI) Kit (1–96) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (97–192) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (193–288) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (289–384) contains 96 pairs of unique dual-indexed PCR primers that can be used to generate up to 96 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.

The Unique Dual Index (UDI) Kit (1–24) contains 24 pairs of unique dual-indexed PCR primers, which are a subset of the Unique Dual Index Kit (1–96), Cat. No. 634752. They can be used to generate up to 24 Illumina-compatible sequencing libraries and are recommended with several of Takara Bio’s RNA-seq and DNA-seq kits.