Takara PrimeSTAR Max DNA Polymerase Ver.2, 400 Rxns

PrimeSTAR Max DNA Polymerase Ver.2 is a B-family polymerase blend that delivers exceptional fidelity and ultra-fast extension speed, making it ideal for applications such as cloning, mutagenesis, and high-throughput PCR. Compared to the original PrimeSTAR Max (Cat. # R045A/B), Ver.2 achieves higher success rates in fast amplifications while maintaining the same trusted accuracy—serving as a fully compatible, superior alternative. Includes a 2X premix that contains all necessary components (enzyme, buffer, dNTP mix) for assay setup. Primers and template not included.

Cat. # R047B contains 4 of Cat. # R047A. Please refer to Cat. # R047A for complete product documentation and resources.

cDNA amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.PrimeSTAR Max DNA Polymerase Ver.2 (Cat. # R047A), PrimeSTAR Max DNA Polymerase (Cat. # R045A) (Panel A), and high-fidelity enzymes from Company N and Company T (Panel B) were used to amplify theTFRgene (4 kb) from cDNA templates obtained from from 10 ng to 1,000 ng of total RNA (50 µl reaction volume) under conditions recommended by the respective manufacturers. PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification of cDNA templates from 200 ng or greater of total RNA at a 5 sec/kb extention time (PanelA, top), while increasing the extension time to 10 sec/kb (Panel A, bottom) resulted in improved amplification for cDNA templates obtained from 400 ng to 1 µg of total RNA.

Genomic DNA amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.PrimeSTAR Max DNA Polymerase Ver.2 (Cat. # R047A), PrimeSTAR Max DNA Polymerase (Cat. # R045A) (Panel A), and high-fidelity enzymes from Company N and Company T (Panel B) were used to amplify theDCLRE1Agene (4 kb) using human genomic DNA (gDNA; 0.1 ng to 1 µg in 50 µl reaction volume) under conditions recommended by the respective manufacturers. PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification of gDNA templates (Panel A, top) and increasing the extension time from 5 sec/kb to 10 sec/kb (Panel A, bottom) resulted in improved amplification, specially for 600 ng to 1 µg of gDNA template.

Long template amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 compared with PrimeSTAR Max DNA Polymerase and high-fidelity enzymes from Company N and Company T.Templates of lengths ranging from 0.5 kb to 15.0 kb were amplified from 100 ng human genomic DNA under conditions recommended by the respective manufacturers in 50 µl reaction volumes. Amplification efficiency of PrimeSTAR Max DNA Polymerase Ver.2 (Cat. # R047A) was compared with PrimeSTAR Max DNA Polymerase (Cat. # R045A) (PanelA) and high-fidelity enzymes from Company N and Company T (PanelB). PrimeSTAR Max DNA Polymerase Ver.2 exhibited the strongest amplification with an extension time of 5 sec/kb for templated under 6 kb and 10 sec/kb for templates over 6 kb. High-fidelity PCR enzymes from Company N and Company T required longer extension times, adding to the total reaction time to achieve comparable amplification (PanelB). The total reaction time for amplifying a 15 kb template is indicated in green for PrimeSTART Max DNA Polymerase Ver.2 and PrimeSTAR Max DNA Polymerase and red for high-fidelity enzymes from Company N and Company T.