Key Specifications Table
| Key Applications | Species |
|---|---|
| CULT, Cult | Human |
| Description | |
|---|---|
| Catalogue Number | SCM151 |
| Description | PLTGold® Human Platelet Lysate |
| Background Information | PLTGold® Human Platelet Lysate is a Xeno-Free cell culture supplement that is a superior alternative to fetal bovine serum (FBS) for use in human mesenchymal stem cell cultures. PLTGold is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax® platelet lysate. Optimal growth of MSCs can be achieved with 5% PLTGold. PLTGold been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine. PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use. |
| Applications | |
|---|---|
| Application | Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 100ml. |
| Key Applications |
|
| Application Notes | Instructions for Use 1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2. 2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use). 3. Do not allow primary MSC confluence to exceed 70-80%. |
| Biological Information | |
|---|---|
| Media Form | Liquid |
| Stem Cell Type |
|
| Product Usage Statements | |
|---|---|
| Quality Assurance | All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis. Each lot is extensively tested for the following parameters: Sterility Testing: Negative for growth pH: 6.8-7.8 Total protein: 4.0-6.5 g/dl Osmolality: 250-330 mOsm/kg Endotoxin USP <85>: <10 EU/mL Mycoplasma: Not detected Cell Growth: Pass |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended. |
| Packaging Information | |
|---|---|
| Material Size | 100 mL |