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Active, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
expressed in E. coli
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_003010.2
Entrez Gene Summary
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Gene Symbol
MAP2K4
MKK4
JNKK
MEK4
MAPKK4
JNKK1
SEK1
SERK1
PRKMK4
Protein Target
MKK4
Purification Method
Purified using glutathione agarose. Activated by incubation with MEKK and then re-purified using Ni2+/NTA agarose
Target Sub-Family
STE
UniProt Number
P45985
UniProt Summary
FUNCTION: SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). SIZE: 399 amino acids; 44288 Da SUBUNIT: Interacts with SPAG9 (By similarity). TISSUE SPECIFICITY: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). SIMILARITY: SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight
67.7kDa
Product Usage Statements
Quality Assurance
routinely evaluated by phosphorylation of ATF2 substrate
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
6 months at -70C
Packaging Information
Material Size
10 µg
Material Package
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size.
