The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Inactive, Recombinant Mouse Mkk4, Corresponding To Amino Acids 35-397, Fused To An N-Terminal Gst-Tag, For Use In Kinase Assays. - Mill (Additional S&H or Hazmat Fees May Apply)
Recombinant mouse MKK4, corresponding to amino acids 35-397, fused to an Nterminal GST-tag
Product Information
Quality Level
MQ100
Applications
Application
Inactive, recombinant mouse MKK4, corresponding to amino acids 35-397, fused to an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
Kinase Assay
Biological Information
Source
expressed in E. coli
Specific Activity
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
NM_003010.2
Entrez Gene Summary
This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Gene Symbol
MAP2K4
MKK4
JNKK
MEK4
MAPKK4
JNKK1
SEK1
SERK1
PRKMK4
Protein Target
MKK4
Purification Method
GSH-agarose chromatography
Target Sub-Family
STE
UniProt Number
P45985
UniProt Summary
FUNCTION: SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1). SIZE: 399 amino acids; 44288 Da SUBUNIT: Interacts with SPAG9 (By similarity). TISSUE SPECIFICITY: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity). SIMILARITY: SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight
65kDa
Product Usage Statements
Quality Assurance
routinely evaluated by phosphorylation of ATF2 substrate
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
