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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IHC, IP, FUNC, WB | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1360 |
| Replaces | CBL1360P |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD117 Antibody, clone ACK2 |
| Alternate Names |
|
| Background Information | This 145 kDa molecule is a member of the tyrosine kinase receptor family and is expressed by a majority of hematopoietic progenitor cells. The interaction of the mouse c-kit receptor and steel factor promotes proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells, and plays a role in signaling and activation of these cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD117 using this Anti-CD117 Antibody, clone ACK2 validated for use in FC, IH, IP, FUNC & WB. |
| Key Applications |
|
| Application Notes | Flow Cytometry: 0.2 μg per 10e6 cells Immunoprecipitation Immunohistochemistry (Frozen) Western blot Blocking Ligand Binding Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | ACK2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | Specifically recognizes mouse CD117, known as c-kit receptor, stem cell factor receptor and steel factor receptor. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). SIZE: 976 amino acids; 109865 Da SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT). SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Stem Cell Type |
|
| Molecular Weight | 145 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. |
| Packaging Information | |
|---|---|
| Material Size | 500 µg |