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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, FC, WB, IHC, FUNC | R | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1366 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD140a Antibody, clone APA5 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin in PBS, 150 mM NaCl, pH 7.2, containing 0.09% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect CD140a using this Anti-CD140a Antibody, clone APA5 validated for use in ELISA, FC, WB, IH. |
| Key Applications |
|
| Application Notes | Flow Cytometry: <= 1 μg per 10E6 cells ELISA Immunohistochemistry: 10μg/mL Blocking of Ligand Binding Western Blotting Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Clone | APA5 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rat |
| Specificity | Specifically recognizes mouse CD140a, the alpha chain of PDGF receptor. A receptor tyrosine kinase, PDGFRa forms dimers on the surface upon ligand binding and phosphorylates substrates. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. |
| Isotype | IgG2aκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P16234 # Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. SIZE: 1089 amino acids; 122670 Da SUBUNIT: Homodimer, and heterodimer with PDGFRB. Interacts with SHB (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DISEASE: SwissProt: P16234 # A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage. SIMILARITY: SwissProt: P16234 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |