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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R | FC, IP, IH(P) | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL1512Z |
| Replaces | MAB1405Z |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Integrin αM [CD11b] Antibody, clone OX-42 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The immunoglobulin was purified by protein G affinity chromatography and is presented as a liquid in phosphate buffered saline containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Integrin αM [CD11b] using this Anti-Integrin αM CD11b Antibody, clone OX-42 validated for use in FC, IH, IH(P) & IP. |
| Key Applications |
|
| Application Notes | Flow cytometry Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15 no post-fix). Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG bead for capture only. The use of protein A or protein G is not recommended. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Rat peritoneal macrophages |
| Clone | OX-42 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3. FUSION PARTNER: NSO myeloma cell line. |
| Isotype | IgG2a |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. SIZE: 1152 amino acids; 127179 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes. DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain in undiluted aliquots at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |