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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, Rb | FC, IP, WB, IHC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL162 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-CD9 Antibody, clone MM2/57 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-CD9 Antibody, clone MM2/57 detects level of CD9 & has been published & validated for use in FC, IP, WB, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: Frozen sections of platelets, monocytes and pre-B cells with differential staining on lymphoid and epithelial tissues Platelet inhibition and aggregation studies Optimal working dilutions must be determined by the end user. the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human platelet glycoprotein |
| Clone | MM2/57 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with a 25 kDa molecular weight band on gels of non-reduced platelet membranes and immunoprecipitates a similar band from Iodine125-labeled platelets. The antigen is expressed on eosinophils, granulocytes, monocytes and numerous stromal cells. This CD9 antigen belongs to the TM4 superfamily of cell surface proteins with the N- and C-terminals localized in the cytoplasm. |
| Isotype | IgG2bκ |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. In addition, the protein appears to promote muscle cell fusion and support myotube maintenance. |
| Gene Symbol |
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| Purification Method | Protein A Purfied |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P21926 # Involved in platelet activation and aggregation. Regulates paranodal junction formation. Required for gamete fusion. Involved in cell adhesion, cell motility and tumor metastasis. SIZE: 228 amino acids; 25416 Da SUBUNIT: Forms both disulfide-linked homodimers and higher homooligomers as well as heterooligomers with other members of the tetraspanin family. Associates with CR2/CD21 and with PTGFRN/CD9P1. Interacts directly with IGSF8. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed by a variety of hematopoietic and epithelial cells. PTM: Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. & Phosphorylated on tyrosine following B-cell activation. SIMILARITY: SwissProt: P21926 ## Belongs to the tetraspanin (TM4SF) family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 1 year at 4°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |