Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1789 |
| Replaces | CBL269 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Migration Inhibitory Factor Related Proteins 8 and 14 Antibody, clone AHN-17 |
| Alternate Names |
|
| Background Information | Protein S100-A8/9 (UniProt: P05109/ P06702; also known as MRP-8/MRP-14, Leukocyte L1 complex light chain/ Leukocyte L1 complex heavy chain, p8/p14, S100 calcium-binding protein A8/A9) are encoded by the S100A8/S100A9 (also known as MRP8/MRP14) genes (Gene ID: 6279/6280) in human. MRP-8 and MRP-14 are calcium- and zinc-binding protein that play a prominent role in the regulation of inflammatory processes and immune response. They are known to induce neutrophil chemotaxis and adhesion. They are predominantly found as calprotectin and perform a variety of intra- and extracellular functions. Their intracellular functions include facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes, and activation of the neutrophilic NADPH-oxidase. They are known to activate NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane. MRP-8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. Their extracellular functions include proinflammatory, antimicrobial, oxidant-scavenging and induction of apoptosis. MRP-14 is reported to induce neutrophil chemotaxis and adhesion and can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 pathways and can induce degranulation of neutrophils by a MAPK-dependent mechanism. MRP-8 and MRP-14 also acts as danger associated molecular pattern (DAMP) molecules and stimulate innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation end (AGER) products. They also play an impotent role as oxidant scavengers and have a protective role in preventing exaggerated tissue damage by scavenging oxidants. (Ref.: Atallah, M., et al. (2012). PloS One. 7(2); e29333; Nakatani, Y., et al. (2005). Mediators Inflamm. 2005(5); 280-292; Ryckman, C., et al. (2003). J. Immunol. 170(6); 3233-3242). |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Migration Inhibitory Factor Related Proteins 8 & 14 using this Anti-Migration Inhibitory Factor Related Proteins 8 & 14 Antibody, clone AHN-17 validated for use in WB & IC. |
| Key Applications |
|
| Application Notes | Immunocytochemistry on ethanol fixed cells Immunoblotting on un-reduced gels Optimal dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Neutrophils |
| Clone | AHN-17 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Migration Inhibitory Factor Releasing Proteins -8 and -14 |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P05109 # Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. SIZE: 93 amino acids; 10835 Da SUBUNIT: Homodimer. SIMILARITY: SwissProt: P05109 ## Belongs to the S-100 family. & Contains 2 EF-hand domains. MISCELLANEOUS: Binds two calcium ions per molecule with an affinity similar to that of the S100 proteins. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |