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Millipore Anti-Collagen Type IV Alpha 2 Chain Antibody, Clone 23iic3
Millipore Anti-Collagen Type IV Alpha 2 Chain Antibody, Clone 23iic3
List Price$616.00Your Price$616.00
Millipore Detect Collagen Type IV Α 2 Chain Using This Anti-Collagen Type IV Α 2 Chain Antibody, Clone 23iic3 Validated For Use In ELISA, Ih(P) & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen
Human amniotic type IV collagen
Clone
23IIC3
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes Human type IV collagen, alpha-2 chains.
Isotype
IgG1
Species Reactivity
Human
Monkey
Rat
Antibody Type
Monoclonal Antibody
Entrez Gene Summary
This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene SO that each gene pair shares a common promoter.
Gene Symbol
COL4A2
Purification Method
Unpurified
UniProt Number
P08572
Molecular Weight
170 kDa
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
