Sigma-Aldrich Kieselguhr F254 25 Tlc Pla

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

200 μm

Plate L × W

20 cm × 20 cm

Particle Size

10-12 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

25 Glassplates 20 x 20 cmPrecoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.

Linkage

Replaces: 5738-7; 5738

Analysis Note

Layer thickness: 180 - 220 µM
Deviation of layer thickness per plate: ≤ 35 µM
Chromatographic testing:
amino acid test
hRf-values
- β-alanine, short-time test:
20 - 30
- L-alanine, amino acid test: 40 - 50
- DL-Valin, amino acid test: 75 - 85
- L-leucine, amino acid test: 88 - 95
Typical value determined on a plate
Eluent: n- propanol/ water (80/20 v/v)