Sigma-Aldrich Silica Gel 60 F254 0.5 mm

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 20 plates

Technique(S)

thin layer chromatography (TLC): suitable

Layer Thickness

0.5 mm

Plate L × W

20 cm × 20 cm

Particle Size

20-40 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

Related Categories

Thin Layer Chromatography (TLC)

General Description

20 Glass plates 20 x 20 cmPLC plates allow users to separate samples that vary greatly in size – from grams down to milligrams. Available with or without a fluorescence indicator, PLC plates has 0.5 mm thickness. They also use our same proven silica-binder technology as in analytical TLC plates. To isolate the substance by extraction, users can simply scrape the spot from the layer.

Application

• An eco-friendly and cost-effective HPTLC method for quantification of COVID-19 antiviral drug and co-administered medications in spiked human plasma: This research developed an environmentally friendly high-performance thin-layer chromatography method to analyze COVID-19 antiviral drugs alongside other medications in human plasma samples, demonstrating effective use of Silica gel 60 F254 plates for clinical applications (Ghozzy et Al., 2024).
• Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in active pharmaceutical ingredient (API) and its dosage forms: This study validates a robust HPTLC method using Silica gel 60 F254 plates to determine tetracycline hydrochloride concentrations in pharmaceutical forms, aiding drug stability and quality control (Gashaw et Al., 2024).
• Robust high-performance thin-layer chromatography (HPTLC) method for stability assessment and simultaneous quantification of epigallocatechin-3-gallate and rosmarinic acid in lipid-based nanoparticles and biological matrices: This publication discusses a reliable HPTLC technique for analyzing complex biological samples and lipid nanoparticles, highlighting the versatility of Silica gel 60 F254 plates in nanomedicine research (Koli et Al., 2024).
• Development and validation of a high-performance thin layer chromatography method for the simultaneous determination of amoxicillin and clavulanic acid combinations in tablet dosage forms: The research introduces a validated HPTLC method for simultaneously detecting amoxicillin and clavulanic acid in tablet forms, utilizing Silica gel 60 F254 plates for pharmaceutical analysis (Asres et Al., 2023).
• Simultaneous analysis of the of levamisole with triclabendazole in pharmaceuticals through developing TLC and HPLC-PDA chromatographic techniques and their greenness assessment using GAPI and AGREE methods: This study provides an analytical method using Silica gel 60 F254 plates to analyze levamisole and triclabendazole, assessing the environmental impact of the chromatographic techniques employed (Attia et Al., 2023).

Linkage

Replaces: 5744-7; 5744

Analysis Note

Layer thickness: 360 - 530 µM
Deviation of layer thickness per plate: ≤ 80 µM
Chromatographic testing:
colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 23
- Ceres Black g, colour test, lipophile: 40 - 46
- ceres Violet brn, colour test, lipophile: 58 - 65
Typical value determined on a conditioned plate
Eluent: toluene (45% rel. humidity)