Sigma-Aldrich Rp-18 F254s 25 Hptlc Plate

Material

glass support
silica gel 60 matrix

Quality Level

100

Feature

binder Organic Polymer
fluorescent indicator

Packaging

pkg of 25 plates

Technique(S)

HPTLC: suitable

Layer Thickness

150-200 μm

Plate L × W

20 cm × 10 cm

Particle Size

5-6 μm

Pore Size

60 Å medium pore diameter

Storage Temp.

2-30°C

General Description

25 Glass plates 20 x 10 cmThese plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples. Our concentrating zone plates are based on different adsorption properties of two adsorbents. The first is a large pore concentrating adsorbent where the samples are applied; the second is a selective layer for separation. Regardless of the spots’ shape, size, or position, the sample always concentrates as a narrow band where the two adsorbents overlap and where the separation starts. These plates allow users to quickly and easily apply any kind of sample, even large volumes of diluted samples.

Linkage

Replaces: 15498-6; 15498

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
layer thickness concentrating zone: 70 - 110 µm
Fluctuation in the thickness of the concentration zone: ≤ 35 µm
Chromatographic testings:
A) cholesterin test
hRf-values
- cholesterin, cholesterin test:
11 - 23
B) Steroide test
- methyltestosterone, steroid test: 20 - 40
- Reichstein S, steroid test: 50 - 70
- hydrocortisone, steroid test: 65 - 85
Typical value determined on a activated plate (120°C, 15 min)
Eluent a) acetone/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)