RPI Ez-96 Dna Methylation-Gold Magprep, 4 X 96 Rxns

The EZ-96 DNA Methylation-Gold MagPrep integrates DNA denaturation and bisuLfite conversion processes into one step followed by a magnetic bead-based clean-up for high-throughput methylation analysis. To streamline the procedure, high temperature is used instead of sodium hydroxide to denature DNA. DesuLphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. The EZ DNA Methylation-Gold kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for downstream analyses, including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.

Complete, high-throughput bisuLfite conversion of GC-rich DNA in less than 3 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines to uracil.
High throughput (96-well), automated desuLphonation and recovery of bisuLfite-treated DNA. Eluted, uLtra-pure DNA is ideal for use in subsequent molecuLar-based analyses.