Cell Signaling Phospho-4e-Bp1 (Ser65) (D9g1q) Rabbit mAb

Name: Cell Signaling Phospho-4e-Bp1 (Ser65) (D9g1q) Rabbit mAb
SKU: CSIG-13443S
Price: 419.15 USD

List Price $419.15 Your Price $419.15

Cell Signaling Phospho-4e-Bp1 (Ser65) (D9g1q) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-13443S

MFG.PART: 13443S

UNSPSC: 41105803

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	15-20
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-4E-BP1 (Ser65) (D9G1Q) Rabbit mAb recognizes endogenous levels of 4E-BP1 protein only when phosphorylated at Ser65.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser65 of human 4E-BP1 protein.
Background	Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).