Cell Signaling S6 Ribosomal Protein (5g10) Rabbit mAb (Hrp Conjugate)

Name: Cell Signaling S6 Ribosomal Protein (5g10) Rabbit mAb (Hrp Conjugate)
SKU: CSIG-14467S
Price: 454.50 USD

List Price $454.50 Your Price $454.50

Cell Signaling S6 Ribosomal Protein (5g10) Rabbit mAb (Hrp Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-14467S

MFG.PART: 14467S

UNSPSC: 41105803

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	32
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	S6 Ribosomal Protein (5G10) Rabbit mAb (HRP Conjugate) detects endogenous levels of total S6 ribosomal protein independent of phosphorylation.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of human S6 ribosomal protein.
Background	One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5 untranslated regions (2). These particular mRNA transcripts (5TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of S6 protein (4,5).

Specifications