Takara Lenti-X Integration Site Analysis Kit \Each

The Lenti-X Integration Site Analysis Kit contains all the reagents needed for PCR-based identification of lentiviral vector integration sites in transduced cell clones. The first step is the construction of pools of adaptor-ligated genomic DNA fragments, referred to as "libraries". These libraries can then be used for identifying genomic DNA sequences flanking the 5 end of the integrated provirus using long-distance PCR.

Flow chart of the Lenti-X integration site analysis protocol.The major steps include construction of three viral integration libraries, PCR amplification of genomic DNA from the libraries, and analysis of PCR products. The gel shows a typical result. Lane 1: HpaI library. Lane 2: DraI library. Lane 3: SspI library. Lane 4: No template control. Lane m: DNA size markers. AP: Adaptor primer. LSP: Lentivirus-specific primer.