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Anti-HA Tag Antibody, clone DW2, rabbit monoclonal
Background Information
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Product Information
Format
Culture Supernatant
Control
HA-tagged WNT-1 protein.
Presentation
Cultured supernatant in 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Anti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
Key Applications
Western Blotting
Application Notes
Western Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.
Biological Information
Immunogen
KLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.
Clone
DW2
Host
Rabbit
Specificity
Recognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.
Isotype
IgG
Species Reactivity
Vertebrates
Species Reactivity Note
Most common vertebrate species tested. Reactivity with other species has not been confirmed.
Antibody Type
Monoclonal Antibody
Molecular Weight
Various
Product Usage Statements
Quality Assurance
Western Blot Analysis Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
