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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H, Rb | ELISA, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | CBL191 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Complement C3a Antibody, clone H13 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-Complement C3a Antibody, clone H13 is validated for use in ELISA, IH, WB for the detection of Complement C3a. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | Human complement component C3 |
| Clone | H13 |
| Host | Mouse |
| Specificity | The antibody recognizes an epitope on the alpha-chain of Complement C3 (Western blot). The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited; similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P01024 # Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. SIZE: 1663 amino acids; 187148 Da SUBUNIT: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha chain). During pregnancy, C3dg exists as a complex (probably a 2:2:2 heterohexamer) with AGT and the proform of PRG2. Interacts with CR2 and VSIG4. SUBCELLULAR LOCATION: Secreted. PTM: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha chain fragment 1 + alpha chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g. DISEASE: SwissProt: P01024 # Defects in C3 are the cause of C3 deficiency [MIM:120700]. It can result in susceptibility to pyogenic infection. & Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. SIMILARITY: SwissProt: P01024 ## Contains 1 anaphylatoxin-like domain. & Contains 1 NTR domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2°-8°C for up to 12 months from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 50 µg |