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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, R, Fg | ICC, IHC, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1567 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Myelin Associated Glycoprotein Antibody, clone 513 |
| Alternate Names |
|
| Background Information | Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Myelin Associated Glycoprotein using this Anti-Myelin Associated Glycoprotein Antibody, clone 513 validated for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Western Blot: 20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003} Immunocytochemistry: 5-10 µg/mL of a previous lot was used, methanol fixation required. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain. |
| Clone | 513 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Detects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. |
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P20916 # Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha2,3- linked sialic acid (By similarity). SIZE: 626 amino acids; 69069 Da SUBUNIT: Binds to RTN4R (By similarity). SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. SIMILARITY: SwissProt: P20916 ## Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. & Contains 4 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
| Molecular Weight | 100 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by immunohistochemistry on rat hippocampus. Immunohistochemistry(paraffin): Representative image from a previous lot. Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -20ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |