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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, R | ELISA, IP, RIA, WB, ICC, IHC, FUNC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1613 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1 |
| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl.pH 7.6 with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Kinesin Antibody, heavy chain, non-neuronal KHC, clone H1 detects level of Kinesin & has been published & validated for use in ELISA, IP, RIA, WB, IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry on paraformaldehyde fixed tissue. Also works for EM. Immunocytochemistry: paraformaldehyde fixed cells; 5-10 RT, freshly made 4% PFA; permeabilize with 0.1-.2% triton X-100 in 3% normal serum blocking buffer Immunoblotting: recognizes 124kDa KHC from non-neuronal samples. Immunoprecipitation Inhibition of kinesin mediated transport EIA/RIA Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain kinesin. |
| Epitope | heavy chain, non-neuronal KHC |
| Clone | H1 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the major form of heavy chain of non-neuronal KHC near the N-terminus. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity). SIZE: 963 amino acids; 109685 Da SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU. SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity). DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at 2-8°C in undiluted aliquots for up to 6 months. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |