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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Ma, Sqd, Su, H, M, R | ELISA, ICC, IHC, IP, FUNC, RIA, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1614 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 |
| Background Information | Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: A previous lot was used on paraformaldehyde fixed tissue. Also works for EM. Immunocytochemistry: A previous lot of this antibody was used in Immunocytochemistry. Immunoprecipitation: A previous lot of this antibody was used in Immunoprecipitation. EIA/RIA: A previous lot of this antibody was used in RIA. Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments. Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Bovine brain kinesin. |
| Epitope | a.a.420-445 |
| Clone | H2 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | Reacts with the heavy chain of kinesin near the N-terminus. |
| Isotype | IgG2b |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
|
| Purification Method | Protein A Purfied |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P33176 # Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity). SIZE: 963 amino acids; 109685 Da SUBUNIT: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 (By similarity). Interacts with SYBU. SUBCELLULAR LOCATION: Note=Uniformly distributed between soma and neurites in hippocampal neurons (By similarity). DOMAIN: SwissProt: P33176 Composed of three structural domains: a large globular N- terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. SIMILARITY: Belongs to the kinesin-like protein family. Kinesin subfamily. & Contains 1 kinesin-motor domain. |
| Molecular Weight | 124 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by Western Blot on Mouse Brain lysates. Western Blot Analysis: 1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates. |
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |