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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Av, Ma | WB, ICC, IHC | M | Affinity Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB1621 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11 |
| Background Information | Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified antibody in PBS, 50% glycerol, and 5 mM sodium azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections Immunoblotting: 1:1000 - 1:2000 ECL detection. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea. |
| Epitope | C-terminus |
| Clone | 3H11 |
| Host | Mouse |
| Specificity | Reacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament. |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. SIZE: 916 amino acids; 102448 Da PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function. SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 300 µL |