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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, Pm | ELISA, FC, IP, ICC, IHC, FUNC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB16983 |
| Replaces | 04-1131 |
| Brand Family | Chemicon® |
| Trade Name |
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| Description | Anti-Integrin α4 Antibody, clone P1H4 |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Purified |
| Presentation | Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.01% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Integrin α4 Antibody, clone P1H4 detects level of Integrin α4 & has been published & validated for use in ELISA, FC, IP, IC, IH. |
| Key Applications |
|
| Application Notes | Immunohistochemistry (preferred fixatives are acetone and alcohols) Immunocytochemistry Immunoprecipitation FACS Analysis ELISA Radioimmunoassay Biological Activity: MAB16983 inhibits CS-1 and VCAM binding. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Clone | P1H4 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | The involvement of integrins in vascular proliferation, adhesion, and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Monoclonal antibody MAB16983 was produced by immunization with human T lymphocytes. The antibody is reactive with integrin a4 from primate and human species. |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Entrez Gene Number |
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| Entrez Gene Summary | The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain. |
| Gene Symbol |
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| UniProt Number |
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| UniProt Summary | FUNCTION: Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. SIZE: 1032 amino acids; 114,900 Da SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. SIMILARITY: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at +2-8C. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |