NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Millipore Detect Actin Using This Anti-Actin Antibody, Clone Jla20 Validated For Use In Western Blotting, Immunofluorescence. - Mill (Additional S&H or Hazmat Fees May Apply)
Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes.
Product Information
Format
Purified
Control
SK-BR3 cell lysate
Presentation
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality Level
MQ100
Applications
Application
Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence.
Key Applications
Western Blotting
Immunofluorescence
Application Notes
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.)
Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)
Biological Information
Immunogen
Chicken cytoskeletal proteins
Clone
JLA20
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Isotype
IgMκ
Species Reactivity
Human
Chicken
Species Reactivity Note
Demonstrated to react with Human. Predicted to react with Chicken based on immunogen design.
Antibody Type
Monoclonal Antibody
Entrez Gene Number
NP_001026234
Gene Symbol
ACTA1
ACTA
UniProt Number
P68139
Molecular Weight
~42 kDa observed
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in SK-BR3 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
